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3 edition of Regulation of the MAP kinase cascade by ACTH in Y1 adrenal cells found in the catalog.

Regulation of the MAP kinase cascade by ACTH in Y1 adrenal cells

Thuyanh Le

Regulation of the MAP kinase cascade by ACTH in Y1 adrenal cells

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  • 8 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1999.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL19262785M
ISBN 100612461432

Pituitary ACTH-dependent Cushing's syndrome caused by ectopic CRF secretion from an intra-adrenal phaeochromocytoma D Woods 1, V Arutchealvam 1, A Madathil 1, R Peaston 2, S Turner 2, RDG Neely 2, J Graham 3, D Richardson 3, TJW Lennard 4, R Bliss 4, SB Bennett 5, . De Wied used a special type of ACTH in these experiments called ACTH This fragment of the complete ACTH molecule does not stimulate the adrenal cortex to produce hormones. Therefore it is unlikely that the restoration of avoidance behaviour in these rats is due to a secondary effect of ACTH on the adrenal cortex. If the address matches an existing account you will receive an email with instructions to retrieve your username.


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Regulation of the MAP kinase cascade by ACTH in Y1 adrenal cells by Thuyanh Le Download PDF EPUB FB2

The involvement of salt-inducible kinase, a recently cloned protein serine/threonine kinase, in adrenal steroidogenesis was investigated. When Y1 mouse adrenocortical tumor cells were stimulated by ACTH, the cellular content of salt-inducible kinase mRNA, protein, and Cited by:   Adrenocorticotropic hormone treatment activates the cAMP–PKA signaling cascade, and we have recently shown that the expression of salt-inducible kinase 1 (SIK1), a serine/threonine kinase that belongs to the stress- and energy-sensing AMPK family of kinases, is also rapidly induced in Y1 adrenal cells in response to ACTH via the cAMP–PKA Cited by: The role of ACTH in stimulating or inhibiting growth of adrenal cells has been a subject of some controversy.

Reports that ACTH may stimulate Erk/MAP kinase in Y1 cells have suggested a role for cAMP in this process. In attempting to extend this work the ACTH responses in the human HR cell line have been studied. ACTH is a potent regulator of gene expression in human adrenal cells Article in Journal of Molecular Endocrinology 45(1) July with 37 Reads How we measure 'reads'.

ACTH stimulates adrenal growth in vivo, whereas in vitro ACTH has an inhibitory effect on adrenal cell proliferation. In serum-starved Y1 cells, a short pulse of ACTH produces a mitogenic effect, which is preceded by the rapid activation of ERK1/2.

This result is in accordance with the requirement of ERK activation for cell by: Mitogen-activated protein kinases (MAPKs) comprise a family of serine/threonine kinases that are activated by a large variety of extracellular stimuli and play integral roles in controlling many cellular processes, from the cell surface to the nucleus.

The MAPK family includes four distinct MAPK cascades, that is, extracellular signal-regulated kinase 1/2 (ERK1/2), p38 MAPK, c-Jun N-terminal Cited by: The modulation of adrenal angiogenesis by ACTH may involve several bioactive signals.

Thrombospondins (TSPs) represent a wide family of extracellular proteins consisting of five members, TSP1–5, which can bind multiple cell surface molecules, including heparin sulfate proteoglycans, low-density lipoprotein receptor-related protein, integrins, CD36, and CD47 ().Cited by: 8.

To determine the role of CaMKK in the regulation of adrenal aldosterone production, we used a CaMKK inhibitor (STO) in HAC15 cells treated with aldosterone agonists (10nM Ang II or 18mM K +).

STO demonstrated dose-dependent inhibition of aldosterone production in 24 hours of treatment with Ang II or K +, at 3μM–20μM and 10μM Cited by: 8. Y1 cells, a short pulse of ACTH prod uces a mitogenic e ect, which is preceded by the rapid activation o f ERK1/2 (39).

is result is in accordance with the r equirement of ERK activation. Cyclic AMP signaling pathway. The cAMP/protein kinase A (PKA) pathway is crucial for the function of the adrenal gland [13, 14] (Figure 1).Corticotropin (ACTH) binds to its G protein-coupled transmembrane receptor (MC2R), leading to the synthesis of cAMP by adenylate cyclase [].

cAMP, acting as a secondary messenger, targets tetramer latter is a cAMP-dependent serine-kinase, consisting Cited by: 9. Y-1 adrenal tumor cells and rat fasciculata cells were shown to possess an enzyme with the properties of protein kinase C.

Activity was stimulated by Ca 2+ and phospholipid (specially phosphatidylserine). Enzyme activity was stimulated by addition of phorbol ester to cell homogenate (ED 50 10 nM) and inhibited by trifluoperazine (ID 50 10 μM). ACTH and cyclic AMP added to Y-1 cells increased Cited by: (1) ACTH stimulates cells to produce low levels of glucocorticoids.

(2) The cells also secrete a constant, low-level of androgens. (a) The androgens produced are weak and physiologically insignificant in men under normal conditions and in women are involved in the maintenance of pubic and axillary hair.

The cell cycle is regulated by a Regulation of the MAP kinase cascade by ACTH in Y1 adrenal cells book kinase in pituitary cells, for example, p21 is an activator of the cell cycle, and p27 and p57 are kinases that inhibit it. Paks are serine/threonine kinases implicated in a number of different intracellular processes, including cytoskeletal regulation and the activation of MAP Cited by: 8.

Although ACTH does play a role in regulation of zona glomerulosa function, angiotensin II is the most important regulator of aldosterone secretion. The zona glomerulosa, like the rest of the adrenal cortex, requires ACTH to prevent atrophy of the tissue.

ACTH can stimulate aldosterone production, but prolonged high levels are Size: KB. Start studying Adrenal Gland Hormones: Physiology of Cortisol: the Major Glucocorticoid in Humans. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

The adrenal cortex is a dynamic organ in which the cells of the outer cortex continually divide. It is well known that this cellular proliferation is dependent on constant stimulation from peptides derived from the ACTH precursor pro-opiomelanocortin (POMC) because disruption of pituitary corticotroph function results in rapid atrophy of the gland.

Reports that ACTH may stimulate Erk/MAP kinase in\ud Y1 cells have suggested a role for cAMP in this process. In attempting to extend\ud this work the ACTH responses in the human HR cell line have been studied.\ud This cell line makes only a very modest cAMP response to ACTH, yet the Erk1/2\ud response is highly reproducible and immediate Author: Mandy Elaine Janes.

The adrenal cortex is the outermost layer of the adrenal gland. Within the cortex are three layers, called "zones". When viewed under a microscope each layer has a distinct appearance, and each has a different function. The adrenal cortex is devoted to production of hormones, namely aldosterone, cortisol, and androgens.

Zona glomerulosa. The outermost zone of the adrenal cortex is the zona Artery: Superior, middle and inferior suprarenal arteries.

The steroidogenic acute regulatory protein (StAR) mediates the rate-limiting and regulated step in steroid hormone biosynthesis, that is, the transfer of the substrate for all steroid hormones, cholesterol, from the outer to the inner mitochondrial membrane [19–25].As such, this protein plays a crucial role in the regulation of steroid hormones required for life itself, in the case of.

Serine/threonine-protein kinases SGK represent a kinase subfamily with orthologs found across animal clades and in yeast (compare Treefam family TF).In most vertebrates, including humans, there are three isoforms encoded by the genes SGK1, SGK2, and name Serum/glucocorticoid-regulated kinase refers to the first cloning of a SGK family member from a cDNA library screen for.

The adrenal cortex produces the glucocorticoid hormones, cortisol and corticosterone, in the cells of its two inner zones, the zona fasciculata and the zona reticularis. These cells also synthesize androgens or male sex hormones, with the main androgen being dehydroepiandrosterone.

Glucocorticoids act on many processes, mainly by altering gene transcription and, thereby, changing the protein. function. These drug leave normal cells and tissues mostly unaffected.

PI3K/Akt/mTOR pathway. The phosphoinositide 3 kinase (PI3K)/ protein kinase B (PKB or Akt)/ mammalian target of rapamycin (mTOR) signaling pathway have been studied in the last years in order to find a more effective cancer therapy.

Abstract. Two hypothalamic peptides which appear to play significant roles in the control of ACTH secretion during stress are the 41 amino acid corticotropin-releasing factor (CRF) 1–4 and vasopressin (AVP) 2– two substances have at least additive, if not synergistic effects in stimulating ACTH release from the anterior pituitary in vivo 5,6 and in vitro 7–Cited by: 5.

Glucocorticoids regulate the proliferation of mouse L cells. Incorporation of [3 H]thymidine is inhibited by % within 24 h after addition of μM effect on L cells is completely reversible.

The expression of the thymidine kinase gene (Tk-1) has been examined in L cells that have been treated with μM dexamethasone for 24 by: Purpose of review Cushing syndrome caused by cortisol-producing adrenal adenomas is a rare condition, associated with high morbidity due to weight gain, diabetes mellitus, osteoporosis, hypertension, muscle weakness, mood disturbance and others.

The first gene to be identified as causative of Cushing syndrome was present an update on protein kinase A (PKA) defects and Cushing. Stimulation of steroidogenesis in the adrenal cortex is a major physiological action of adrenocorticotropic hormone (ACTH).

This action is presumed to be mediated by the ACTH receptor and is functionally connected with the hypothalamus-pituitary-adrenal axis. To gain information concerning the distribution of the ACTH receptor in this axis, we examined mRNA for the ACTH receptor in the Cited by: The Adrenal Cortex Using cholesterol as the starting material, the cells of the adrenal cortex secrete a variety of steroid hormones.

These fall into three classes: glucocorticoids (e.g., cortisol) ; mineralocorticoids (e.g., aldosterone) ; androgens (e.g., testosterone). Production of all three classes is triggered by the secretion of ACTH from the anterior lobe of the pituitary.

Regulation of cortisol secretion HTh CRH Anterior Pituitary ACTH Adrenal cortex from BIO at University of San Francisco.

Cells of the Zona Reticularis are arranged in an inter-connecting network of cords giving the impression of a reticulum and thus the zone's namesake. Cells of this zone synthesizes "Adrenal Androgens": Named as such because they are androgens that are derived from the adrenal cortex and not from the gonads.

Cortisol / Adrenal Dysregulation / ACTH During stressful times, cortisol will increase blood glucose levels and convert fat cells into sugar so the body has a greater ability to cope with stressful situations. This is why the cortisol hormone is often referred to as the “stress hormone”.

in the proliferating cells of the small intestinal crypts We have shown previously that the intestinal epithelium is an important source of extra-adrenal glucocorticoids and that the regulation of glucocorticoid synthesis in the intestinal epithelium is substantially different from that in the adrenal glands (5, 9).

Interestingly, we found that theCited by: Adrenal cortex Hormones Mineralocorticoids Glucocorticoids Addison disease from BIOL at Carthage College.

Reject 2, see third link (adrenal medulla is inside, but ACTH affects the adrenal cortex - outer layer of adrenal gland. Reject 3, because ACTH is secreted by the anterior lobe of the pituitary gland, not the posterior lobe. Answer 3 is also incomplete, because T cells etc.

also produce ACTH. and six day ACTH stimulated rabbit adrenal microsomes, A 53, D protein band was found to increase % in two day, % in four day and % in six day ACTH stimulated microsomes as com­ pared to controls. ACTH stimulation was confirmed by 17a-hydroxy-Cited by: 2.

Background: Serum- and glucocorticoid-inducible kinase 3 (SGK3) plays a pivotal role in Akt-independent oncogenic signaling. Results: HspCdc37 chaperone complex acts in concert with the hydrophobic motif of SGK3 to regulate SGK3 stability and activation.

Conclusion: HspCdc37 complex is essential for SGK3 stability and activation. Significance: The involvement of HspCdc37 in SGK3. PKA, a cAMP-dependent serine–threonine kinase, is perhaps the best characterized protein kinase and provides a clear example of allosteric regulation.

35 In its inactive state, it is a Cited by: Infiltration of adrenal tissue by mononuclear cells has been noted in histologic sections from up to 15 percent of patients with Cushing's syndrome due to diffuse and nodular adrenal hyperplasia Cited by: Acute Adrenal Crisis: Acute life-threatening adrenocortical insufficiency with markedly reduced serum cortisol, which arises in a background of chronic adrenocortical insufficiency (Addison’s disease).

Aetiology Infections, trauma, adrenal haemorrhage, tuberculosis, surgery, dehydration with salt deprivation, destruction of pituitary gland or. The regulation of MAPKs in Y1 mouse adrenocortical tumor cells Endocrinology 10 2-s /en 82 Ferreira J.

Cruz C. Neves D. Pignatelli D. Increased extracellular signal regulated kinases phosphorylation in the adrenal gland in response to chronic ACTH treatment Journal of Endocrinology corticotropin-releasing hormone receptor 1 conditional knockout; extracellular signal-regulated kinase 1/2; mitogen-activated protein kinase; Corticotropin-releasing hormone (CRH) is a aa peptide that exerts a key role in the adjustment of neuroendocrine and behavioral adaptations to alamic CRH neurons drive both basal and stress-induced hypothalamicpituitary-adrenal axis (HPA.

The regulation of the Raf-MEK-ERK cascade has been studied extensively and is one of the best understood MAP kinase cascades (7, 8). MEK directly phosphorylates ERK on a conserved T X Y motif between the kinase subdomain VII and VIII and activates ERK (9).The phosphoinositol-3 (PI 3)-kinase pathway plays a pivotal role in the stimulation of mast cells.

Previous studies have shown, that PI3-kinase is required for mast cell activation and degranulation. Kinases activated through the PI 3-kinase pathway include serum- and glucocorticoid-inducible kinases 1 .Cell cycle-dependent regulation of extra-adrenal glucocorticoid synthesis in murine intestinal epithelial cells.

In: The FASEB Journal. In: The FASEB Journal. 22 (12), pp.